課程資訊
課程名稱
生物電子顯微鏡技術學
Biological Electron Microscopy 
開課學期
101-2 
授課對象
生命科學院  生命科學系  
授課教師
黃玲瓏 
課號
EEB5043 
課程識別碼
B44 U1550 
班次
 
學分
全/半年
半年 
必/選修
選修 
上課時間
星期二2,3(9:10~11:10) 
上課地點
生科419 
備註
初選不開放。正課為23,1、4、@堂為實習,須先與老師聯絡。與陳香君合開
總人數上限:12人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1012LSEM 
課程簡介影片
 
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課程概述

1) Introduction Orientation
2) Preparation for TEM I Sample Fixation & Embedding, Block Trimming
3) Specimen Preparation for TEM II Supporting Film & Glass Knife Making
4) Ultramicrotomy Ultramicrotomy I
5) Section Staining Ultramicrotomy II
6) Principle of EM Section Staining
7) Structure and Operation of TEM Structure and Operation of TEM
8) Cytochemistry & Immunomicroscopy Negative Staining, Shadow-Casting Technique & Lab
9) Scanning Electron Microscopy Analytical Electron Microscopy & Light Micrography
10) Cryo-EM Techniques Freeze-Etching Technique
11) Mid. Examination Cytochemistry & Immunomicroscopy
12) SEM Sample Preparation Darkroom Technique
13) Image Presentation and Analysis Scanning Analytical Electron Microscopy
14) Fluorescent Microscopy Confocal Microscopy & Campus EM Centers
15) Perspectives of Electron Microscopy Academia Sinica EM Centers
16) Presentation of EM-Lab Data Individual assistance to student projects
17) Final Examination
18) Hand in Report
 

課程目標
熟悉各種生物電顯相關技術與原理 
課程要求
 
預期每週課後學習時數
 
Office Hours
 
指定閱讀
 
參考書目
 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
2/19  Lec:Introduction.
Lab:Orientation 
第2週
2/26  Lec:Specimen Preparation for TEM. Lab:Sample Fixation, Embedding and Block Trimming 
第3週
3/5  Lec:Ultramicrotomy.
Lab:Supporting film & Glass knife making 
第4週
03/12  Lec:Section staining & Negative staining. Lab:Ultramicrotomy I 
第5週
03/19  Lec: Principle of EM. Lab: Ultramicrotomy II  
第6週
03/26  Lec: Structure and Operation of TEM. Lab: Structure and Operation of TEM 
第7週
04/02  Lec: Scanning electron microscopy.
Lab: Section staining & Negative staining 
第8週
04/09  Lec: Analytical electron microscopy. Lab: Analytical electron microscopy 
第9週
04/16  Lec:Discussion. Lab:SEM Preparation. 
第10週
04/23  Lec: Cryo-Electron Microscopy. Lab: SEM Observation 
第11週
04/30  Lec: Immuno-Electron Microscopy. Lab:TM-3000 
第12週
05/07  Lec:Image interpretation. Lab: Immunomicroscopy 
第13週
05/14  Lec: Image Processing and analysis. Lab: Individual assistance to student project 
第14週
05/21  Lec: Perspectives of Electron Microscopy. Lab: Individual assistance to student project 
第15週
05/28  Lec: Individual assistance to student project. Lab: Individual assistance to student project 
第16週
06/04  Academic Sinica EM Centers  
第17週
06/11  Presentation of EM-Lab Data (Oral; PPT) 
第18週
06/18  Hand in Report